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Department of Bio-Technology
 
 

Lab Facilities

No. of Experiments in each Laboratory

Semester- I

  • Biochemistry lab - 9
  • Cell biology & Microbiology lab - 13                           
  • Molecular biology &Genetic Engineering lab - 11
  •  Down Stream processing  lab - 11
  • Bioinformatics lab - 6

Semester- II

  • Bio Process Engineering lab - 6
  •  Instrumental Methods Analysis lab - 8
  •  Plant Tissue culture lab - 6
  •  Immunology lab - 9


Laboratory Equipments and List of Experiments



S.No Name of the – LAB No of experiment can be conducted Major equipment
1 Cell biology & Microbiology 13 Laminar Air flow
Autoclave
Rotary shaker
BOD – incubator
Hot air oven
2 Biochemistry 09 Hot air oven
Electronic balance
3 Downstream processing 11 Laminar Air flow
Autoclave
4 Molecular biology and
Genetic Engineering
11 PCR Thermal cycler
Horizontal Electrophoresis kit
Vertical Electrophoresis kit
U.V.Visible Spectrophotometer
U.V.Transilluminator
Gel Dryer
Weighing balance
Microfuge
5 Instrumental method of analysis 08 HPLC
Dry bath
Spectrophotometer
6 Bioprocess and engineering 06 Bioreactor
Ultra freezer
Incubator


Advanced Instruments in The Department

Computer Controlled Bio Reactor

  • Used for demonstration to B.Tech students and for carrying out Project work.
  • Various projects like, alcohol production, enzyme production, amino acids production etc., can be carried out.

HPLC

  • Used for separation ,purification and estimation of proteins and enzymes.

UV-Vis Spectro photo meter

  • Used to measure concentration of Biomolecules like DNA, proteins and organic acids.

Major Equipments

  • Costing more than Rs.5,00,000 - 02
    (HPLC, Bioreactor)
  • Costing more than Rs.50,000 - 12
    (UV-Vis spectrophotometer, PCR, Laminar Air flow, Microscope, -20oC Deep freezer, Electronic balance, Dry water bath, centrifuge, incubator, hot air oven, electrophoresis, micropipettes, autoclave)
  • Computer Facilities - 03

New Lab Proposals



Facility Details NO. Justification
Equipments Electronic balances 2 To weigh the chemicals it is required. This  equipment is required to conduct the experiments as per the syllabus
  Microscope(binocular, simple) 3 To observe the micro organisms.
  Cold centrifuge 1 For the separation of different molecules based on the density at 4oC
  Millipour water purifier 1 To sterilize the water
  Refrigerator 2 To store the chemicals& cultures
  Incubator and shaker 1 To incubate the cultures under stirring conditions
  Compound microscope 6 To observe the micro organisms
  Lyophilizer 1 For preserving the culture
  PH meter 2 To estimate the PH values
  Spectrofluorimeter 1 To measure the concentration of molecules
  Distillation unit 3 To produce distilled water
Equipments Computerized Gel doc 1 To visualize the bands after completion of electrophoresis
  Laminar airflow 1 To maintain aseptic condition
  Autoclave 1 For the sterilization
  Digital PH meter 2 To estimate the PH values
  Hot plate with stirrer 1 To maintain uniform suspension
  Hot air oven 1 To maintain specific temperature
  Computers 30 To perform bioinformatics experiments
  Printer cum scanner 1 To take the printouts
  Furniture   To establish new bioinformatics lab to place the systems
  Tray Dryer 1 For purification the Fermented products
  Floatationcell 1 To separate undesired products from the Fermented products
  Air conditions 8 To maintain the specific temperature for culture room, bio informatics lab
Equipments Waterproff PC tester 1 To estimate the water conductivity
  Green house hood 1 Incubation of the plants
Glass ware      
  Separating Funnel 15 For Filtration
  Micropippets 8 For measuring the low volume of samples
  Micro filters 1 For Filtration
  Test tube holding stands 4 To hold the test tubes
  Culture tubes 250 To culture the organisms
  Culture flask vessels 20 To culture the organisms
  Pipetts 40 For measuring the solutions
  Measuring cylinders 5 For measuring the solutions
  Beakers 20 To store the solutions
  Petri dishes 40 To culture the microbes
  Microtome 1 To cut the thin slices
Teaching Kits Immunology 10 To carry out the experiments in immunology lab
  Miscellaneous(Like scissors syringes needles , aluminium foils, cotton rolls etc)   Supporting ancillary materials for smooth conducting of experiments

 

 
 
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